Basavaraj M. Vastrad1 and Shivayageeswar E. Neelagund2
1Department of Pharmaceutical Biotechnology, S. E. T`s College of Pharmacy, Dharwad, Karnataka, India.
2Department of PG Studies and Research in Biochemistry, Jnana Sahayadri Kuvempu University, Shankarghatta, Karnataka, India.
Abstract
Tetracycline belongs to one of the extracellular produced polyketide antibiotics having hydrophilic properties. Statistical based optimization, Plackett–Burman design (PBD) and response surface methodology (RSM) were occupied to screen and optimize the medium conditions for the production of tetracycline from Streptomyces aureofaciens NCIM 2417, using solid state fermentation. pineapple peel waste was used as both the curious solid support and carbon and nitrogen sources and inorganic salts for the growth of Streptomyces aureofaciens NCIM 2417. Based on the positive influence of the half normal plot obtained from PBD on tetracycline production, three medium conditions – peanut meal, incubation period and soluble starch were screened. Central composite design (CCD) was occupied using these three medium conditions at five levels, for advance optimization, and the second order polynomial equation was derived, based on the experimental data. Response surface methodology showed that the conditions of peanut meal (0.4%), incubation period (day 2) and soluble starch (2%) were the optimal levels for maximal tetracycline production (17.98 mg/g substrate) which were validated through experiments.
Keywords
Streptomyces aureofaciens NCIM 2417, solid state fermentation, pineapple peel, Placket Burman design, central composite design
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