Bioscience & Engineering: An International Journal (BIOEJ)

ISSN : 2349- 848X

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Submission Deadline : December 29, 2018

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ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF ALGERIAN POPULUS NIGRA L. BUDS EXTRACTS

DJAMILA MERGHACHEA* , ZAHIA BOUCHERIT-OTMANIA, IMAD EL HACIB, F , SALIMA MERGHACHEC , ILYAS CHIKHID , AND KEBIR BOUCHERITA, E

a Antibiotics, Antifungal: Physical Chemistry, Synthesis and Biological Activity

Laboratory, Department of Biology, Faculty of Natural sciences and life sciences and earth and the universe.

University of Tlemcen, P. O. Box 119, Tlemcen 13000, Algeria.

b

Laboratoire des Produits Naturels (LAPRONA), University of Tlemcen, Algeria;

c

Laboratoiry of inorganic chemical and environnement (LCIE) University of Tlemcen,

Algeria;

d

Laboratory of Natural and Bioactive substances (LASNABIO), University of Tlemcen,

Algeria.

e

University center of Ain Tmouchent, Algeria, 46000, Algeria.

f

Centre de Recherche Scientifique et Technique en Analyses Physico – Chimiques

C.R.A.P.C

BP 384, Zone Industrielle Bou-Ismail RP 42004 Tipaza, Algeria

ABSTRACT

This study is part of a goal to investigate chemical composition, antibacterial, antifungal and antioxidant activities of the flower buds extracts from the Algerian Polulus nigra L., which were collected from Djarifet - mansourah at Tlemcen city in the West Northern of Algeria. In organic extracts, tanins, flavonoïds, coumarins, alkaloids and terpenoïds were the principals secondary metabolites identified from the flower buds of black poplar. Antibacterial and antifungal activities of extracts were tested using agar-well diffusion method and micro-well determination of MIC assay against eleven bacteria and two Candida species. It was found that extracts of black poplar buds exhibit antibacterial and anticandidal activities with agar disk diffusion (7 to 43mm) and MIC methods (MIC= 90.33 µg/ml against several strains of bacteria and MIC=45.16 µg/ml against Candida albicans). The antioxidant effect of hydroalcoholic extract was evaluated using DPPH and FRAP assays. It was showed good and similar activity than ascorbic acid and BHA by DPPH method: IC50= 220µg/mL for hydroethanol extract.

KEYWORDS:

Populus nigra L. extracts, Antibacterial and antifungal activities, DPPH antioxidant power, Ferric reducing antioxidant power assay (FRAP).